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Volume 9, Part 2 (2001)

  • D.G. Kim. Distribution and population dynamics of Meloidogyne arenaria on oriental melon (Cucumis melo L.) under greenhouse conditions in Korea, 61-68
  • S. Boström and O. Holovachov. Study of Teratocephalidae (Nematoda) from the Ukraine. II. Description of a population of Teratocephalus tenuis Andrássy, 1958 with a discussion of male sexual characters, 69-76
  • L. Barsi and F. Lamberti. Morphometric variation and juvenile stages of Longidorus piceicola Liskova et al., 1997 (Nematoda: Longidoridae) from the former territory of Yugoslavia, 77-83
  • R. Crozzoli and F. Lamberti. Known and new species of Mesocriconema Andrássy, 1965 (Nematoda: Criconematidae) from Venezuela, 85-105
  • N. von Mende. In vitro optimisation of Meloidogyne infections on Arabidopsis thaliana 107-111
  • V.G. Gagarin and O. Holovachov. The genus Tridentulus Eyualem & Coomans, 1995 with description of Tridentulus palustris sp. n. from the Ukraine (Nematoda: Monhysterida) and a key to the species, 113-117
  • V.V. Yushin and S.E. Spiridonov. Ultrastructure of sperm development in Heth mauriesi Adamson, 1982 (Rhigonematida: Hethidae), 119-126
  • T.V. Rubtsova, S.A. Subbotin, D.J.F. Brown and M. Moens. Description of Longidorus sturhani sp. n. (Nematoda: Longidoridae) and molecular characterisation of several longidorid species from Western Europe, 127-136
  • R. Jin, B. Sipes and D. Borthakur. Reproduction of Heterodera schachtii on Bt-transgenic cabbage 137-138
  • M. Radivojevic, D. Krnjaic, S. Krnjaic, J. Bacic, S.A. Subbotin, M. Madani and M. Moens.  Molecular methods confirming the presence of Globodera rostochiensis (Wollenweber, 1923) in Yugoslavia, 139-141
  • Book review, 142
  • Abstracts of the Fourth English Language International Symposium of the Russian Society of Nematologists (dedicated to 110th anniversary of Professor A.A. Paramonov, Russia, Moscow, 11th-14th June, 2001), 143-168

  • Kim, D.G.

    Distribution and population dynamics of  Meloidogyne arenaria on oriental melon (Cucumis melo L.) under greenhouse conditions in Korea

    Summary:
    Oriental melon, Cucumis melo L., grafted on Shintozoa (Cucurbit maxima x Cu. moschata) was transplanted in February in a plastic tunnel inside a greenhouse infested with Meloidogyne arenaria. Population dynamics of M. arenaria juveniles (J2) were monitored at monthly intervals for 12 months, and horizontal and vertical distribution of root systems and number of J2's in soil were examined in July. Soil J2 population dynamics displayed a single peak; nematode density increased slightly after planting but declined to 9/100 cm3 soil at the 70th day after planting to increase again at the 100th day and this continued until the 154th day to reach 3,817/100 cm3. Thereafter, the population declined until December. Juveniles were distributed relatively uniformly horizontally over the 180 cm wide row. Maximum root length occurred at 0 to 25 cm depth, and maximum root weight occurred in the top 5 cm of soil around the row center. The highest density of M. arenaria J2's was at 0 to 25 cm  soil depth, and only a few occurred at 40 to 50 cm. This study of M. arenaria on oriental melon under an intensive greenhouse cultivation system in Korea provides information for better understanding of root-knot nematode biology and population dynamics..

    Key words: distribution, ecology, oriental melon, peanut root-knot nematode, root biomass.


    Boström, S. and Holovachov, O.

    Study of Teratocephalidae (Nematoda) from the Ukraine. II. Description of a population of Teratocephalus tenuis Andrássy, 1958 with a discussion of male sexual characters

    Summary:
    A population of Teratocephalus tenuis Andrássy, 1958 from the Ukraine is described from studies by light and scanning electron microscope. The specimens examined agree well with the original description of T. tenuis but have wider ranges of body length (490-587 vs 526-565 µm), c´-ratio (5.3-8.4 vs 6.0-6.3) and annule width (1.4-1.9 vs 1.6 µm), and differ by length of spicules (15-18 vs 12-13 µm), structure of the cuticle (with vs without longitudinal striae), and the number of genital papillae in the male. The specimens at hand were also compared with populations of T. lirellus Anderson, 1969 from which they differ by a larger c-ratio (7.6-10.1 vs 2.6-5.8), and a smaller c´- ratio (5.3-8.4 vs 8-35). Male sexual characters of T. bisexualis, T. rugosus and T. tilbrooki were re-examined. The taxonomic value of male sexual characters for identification of bisexual species of Teratocephalus is discussed.

    Key words: morphology, scanning electron microscopy, taxonomy, Teratocephalus, T. bisexualis, T. lirellus, T. rugosus, T. tenuis, T. tilbrooki .


    Barsi, L. and Lamberti, F.

    Longidorus piceicola Liskova et al., 1997 (Nematoda: Longidoridae) from the former territory of Yugoslavia

    Summary:
    Longidorus piceicola is reported for the first time from the former territory of Yugoslavia. Morphometric variability of females, males and four juvenile stages are presented.

    Key words:  distribution, Longidorus piceicola, morphometrics.


    Crozzoli, R and Lamberti, F.

    Known and new species of Mesocriconema Andrássy, 1965 (Nematoda: Criconematidae) from Venezuela

    Summary:
    Six previously reported, and four undescribed, species of Mesocriconema Andrássy, 1965 are described from Venezuela. Additional morphometric information is provided on M. dherdei (De Grisse, 1967) Loof & De Grisse, 1989; M. onoense (Luc, 1959) Loof & De Grisse, 1989; M. oostenbrinki (Loof, 1964) Andrássy, 1965; M. ornatum (Raski, 1959) Loof & De Grisse, 1989; M. sphaerocephalum (Taylor, 1936) Loof & De Grisse, 1989 and M. xenoplax (Raski, 1952) Loof & De Grisse, 1989. M. dherdei and M. xenoplax constitute new records from Venezuela. M. apurense sp. n. resembles M. onoense and M. paronostris (Deswal & Bajaj, 1987) Ebsary, 1991; M. juliae sp. n. with large submedian lobes is similar to seven other species within the genus; M. malagutii sp. n. is similar to M. ornatum and M. curvatum (Raski, 1952) Loof & De Grisse, 1989; and M. theobromae sp. n. is similar to M. irregularis (De Grisse, 1964) Loof & De Grisse, 1989, M. axeste (Fassuliotis & Williamson, 1962) Loof & De Grisse, 1989 and M. solivagum (Andrássy, 1962) Loof & De Grisse, 1989. A dichotomous identification key to the species of Mesocriconema occurring in Venezuela is provided.

    Key words: description, dichotomous key, Mesocriconema, M. apurense sp. n., M. juliae sp. n., M. malagutii sp. n., M. theobromae sp. n., Venezuela.


    von Mende, N.

    In vitro optimisation of Meloidogyne infections on Arabidopsis thaliana

    Summary:
    The growth conditions of Meloidogyne incognita on Arabidopsis thaliana were optimised by examining the type of medium and agar, agar concentration and volume applied, and pH and sucrose concentration in the medium. The media which supported high rates of invasion and nematode development were 'Gamborg's B5', 'M&S' (0.1x), 'Knop' (0.2x) and 'White's', independent of the addition of vitamins. More nematodes invaded roots of A. thaliana and developed in media containing 1.5% sucrose with a pH of 6.0. Loading 8 ml of a 0.8% agar medium into 9 cm i.d. Petri dishes gave the best agar thickness and concentration to support root and nematode growth. There were no differences in gall development when using 'Daichin' agar or purified agar.

    Key words: Bagar, Arabidopsis, invasion, pre-galling, root knot nematodes, sucrose concentration.


    Gagarin, V.G. and Holovachov, O.

    The genus Tridentulus Eyualem & Coomans, 1995 with description of Tridentulus palustris sp. n. from the Ukraine (Nematoda: Monhysterida) and a key to the species

    Summary:
    Six females and four males of a new free-living nematode species, Tridentulus palustris sp. n., were found during a nematological study of different habitats of the Shutsk National Natural Park, Ukraine. The new species is most similar to T. brzeskii Gagarin & Gusakov, 2000 from which it differs by having a shorter outer labial setae, more anteriorly situated amphidial fovea and males present. Brief morphometric characteristics of five valid species and a key to the species of the genus Tridentulus are given. Tridentulus obscurus Gagarin, 2001 is synonymized with T. floreanae Eyualem & Coomans, 1995.

    Key words: dichotomous key, Monhysteridae, Nematoda, new species, swamp, Tridentulus palustris, Ukraine.

     


    Yushin, V.V. and Spiridonov, S.E.

    Ultrastructure of sperm development in Heth mauriesi Adamson, 1982 (Rhigonematida: Hethidae)

    Summary:
    Spermatogenesis in the nematode Heth mauriesi (Rhabditia, Rhigonematida, Hethidae), which inhabits diplopod hind gut, was studied by using transmission electron microscopy. During the late stages of development spermatocytes have voluminous cytoplasm filled with ribosomes, numerous cisternae of RER, Golgi bodies, and complexes of aberrant organelles comprised of membranous organelles and fibrous bodies (MO-FB complexes). Each complex includes paracrystalline FB associated, at one end, with MO reduced to a simple flattened cistern. Mature spermatids contain a condensed amorphous nucleus surrounded by mitochondria and MO-FB complexes and synthetic components of the cell are segregated into the residual body. The immature spermatozoon is a highly elongated and clearly polarized cell. The central longitudinal axis of the sperm cell is occupied by the long thread-like nucleus. The plasmalemma of the anterior part is smooth, but forms numerous deep infoldings that protrude into the transparent cytoplasm with rare mitochondria, lipid droplets and sparse filamentous material. The plasmalemma of the posterior part of the spermatozoon is arranged into short and wide microvilli of uniform size. Here, peripheral cytoplasm contains numerous spindle-shaped FB orientated along the longitudinal axis of the cell. The layer of small mitochondria positioned under the FB surrounds the central transparent cytoplasm that is filled with sparse filamentous material. MO, or their derivatives, were not observed in immature sperm. The pattern of sperm development in Heth mauriesi is similar to that described for other nematodes of the subclass Rhabditia, but differs significantly from the spermatogenesis in oxyurids.

    Key words: nematode parasites of diplopods, sperm, spermatogenesis, ultrastructure.


    T.V. Rubtsova, T.V., Subbotin, S.A, Brown, D.J.F. and Moens, M.

    Description of Longidorus sturhani sp. n. (Nematoda: Longidoridae) and molecular characterisation of several longidorid species from Western Europe

    Summary:
    During a nematological survey of orchards in Belgium and vineyards in Germany several populations of an undescribed Longidorus species were found. These populations are described as L. sturhani sp. n. The new species is characterised by a medium body length (4.4-6.4 mm), a slightly expanded and anteriorly flattened head region, symmetrically bilobed pocket-shaped amphids, conoid tail 35-49 µm long, and absence of males. The new Longidorus species is most similar to L. seinhorsti and L. artemisiae. The type population of L. sturhani sp. n. did not transmit virus in bait-tests. Analyses of sequences of D2-D3 regions of the large subunit of rDNA of L. sturhani sp. n. and its position on phylogenetic trees prepared with several other longidorids from Western Europe and New Zealand are presented. 

    Key words: Belgium, evolution, Germany, 28S gene, morphometrics, orchards, Paralongidorus, rDNA, vineyard.