Volume 7, Part 1 (1999)
Slobodyanyuk. Revision of the species Psyllotylenchus pawlowskyi(Kurochkin,
1960) Poinar & Nelson, 1973. II. Description of Kurochkinitylenchus
laevicepsi gen. n., sp. n. and Spilotylenchidae fam. n., 1-18
P. and J.R. Finney-Crawley. A survey of nematode fauna associated
with some marine algae in Newfoundland, 19-28
- W. M.
Wouts and D. Sturhan. Mesocriconema hymenophorum sp. n. (Nematoda:
Criconematidae) from forest soil in Germany, 29-32
- T. V.
Rubtsova, V.N. Chizhov and S.A. Subbotin. Longidorus artemisiae
sp. n. (Nematoda: Longidoridae) from roots of Artemisia sp., Rostov region,
Spiridonov. and M. Moens. Two previously unreported species of steinernematids
from woodlands in Belgium, 39-42
Sturhan. and L. Ruess. An undescribed Steinernema sp. (Nematoda: Steinernematidae)
from Germany and the Scandinavian Subarctic, 43-47
Spiridonov, W.M. Hominick and B.R. Briscoe. Morphology of amoeboid
cells in the uterus of Steinernema species (Rhabditida:
Subbotin, P.D. Halford and R.N. Perry. Identification of populations
of potato cyst nematodes from Russia using protein electrophoresis, rDNA-RFLPs
and RAPDs, 57-63
- A. Adiko and S.R. Gowen. Effect of spores of Pasteuria penetrans
on the motility of second-stage juveniles of Meloidogyne incognita, 65-66
- L.M. Dzhuraeva. Nematode types in the collection of the Institute
of Zoology and Parasitology of the Academy of Sciences of Republic of
Revision of the species Psyllotylenchus pawlowskyi
1960) Poinar & Nelson, 1973. II. Description of Kurochkinitylenchus
laevicepsi gen. n., sp. n. and Spilotylenchidae fam. n.
A new genus and species is proposed as the result of a revision of
the species Psyllotylenchus pawlowskyi sensu (Kurochkin, 1960) Poinar
& Nelson, 1973, which was found to represent three distinct species.
A description of morphology and life-cycle of Kurochkinitylenchus laevicepsi
gen. n., sp. n., a parasite of Nosopsyllus laeviceps fleas, is given.
The nematode has alternation of two parasitic heterosexual generations
during its life-cycle. Peculiarities in the life-cycle of the new genus
correspond to the diagnosis of the subfamily Parasitylenchinae, but morphological
features of the new genus do not coincide with contemporary diagnosis of
this subfamily. New genus can be distinguished from other genera of Parasitylenchinae
by dorsal curvature of the female body, stylet morphology (strong basal
thickening but without knobs), absence of cephalic cone, well separated
spermatheca and several other features. An analysis of the phylogenetic
relationships of Kurochkinitylenchus gen. n. with other genera of
the superfamily Sphaerularioidea is presented. Morphological comparison
is made with the genera Spilotylenchus (Contortylenchinae), Psyllotylenchus,
Incurvinema (Heterotylenchinae, Parasitylenchidae) and Rubzovinema
(Phaenopsitylenchidae). A new family Spilotylenchidae fam. n., new subfamilies,
Spilotylenchinae subfam. n., Psyllotylenchinae subfam. n., Rubzovinematinae
subfam. n. and Kurochkinitylenchinae subfam. n., are proposed.
Key words: Kurochkinitylenchus laevicepsi gen. n.,
sp. n., fleas, morphology, biology, phylogenetic relationship.
P. and Finney-Crawley, J.R.
A survey of nematode fauna associated with some marine
algae in Newfoundland.
Nematodes associated with 19 genera of marine algae on the Avalon Peninsula
of Newfoundland were studied. Forty-seven species of nematodes in 31 genera
were identified. Most nematodes were found on Lithothamnium glaciale,
spp. I & II, Pilayella littoralis and Dictyosiphon foeniculaceus.
Other algae had fewer nematodes, while none were found on five species
of brown algae. Each algal growth form was dominated by different nematode
species. The calcareous, filamentous and shrub-like algae had the greatest
whereas the tuft-like algae and those with prominent blades had the smallest
species diversity, respectively. Epigrowth feeders and predators were the
dominant nematode feeding types found on all algal groups. The difference
in algal structure provided varying degrees of shelter, food and refuge
from predators and therefore contributed to patterns of nematode abundance
Key words: marine algae, nematodes, diversity, abundance
Wouts, W.M. and Sturhan, D.
Mesocriconema hymenophorum sp. n. (Nematoda: Criconematidae)
from forest soil in Germany
Specimens of Mesocriconema hymenophorum sp. n. were recovered
from soil collected in a mixed forest in Germany, in which spruce was predominant.
The species is characterised by a distinct hyaline sheet around the body,
as also occurs with M. axeste, M. basili and M. longistyletum.
The new species differs from M. axeste and M. basili by having
a longer stylet in females (94-105 µm vs 51-60 µm and 68-74
µm, respectively), and from M. longistyletum by the relatively
small, vs very large, submedian lobes in the lip region and the straight,
vs sigmoid, vagina.
Key words: Criconematidae, description, Germany, Mesocriconema
hymenophorum sp. n., Nematoda, new species, pine-wood
T.V., Chizhov, V.N. and Subbotin, S.A.
Longidorus artemisiae sp. n. (Nematoda: Longidoridae)
from roots of Artemisia sp., Rostov region, Russia
A description of Longidorus artemisiae sp. n., a bisexual species
associated with Artemisia sp. near Starocherkassk, Rostov region, South
part of European Russia is provided. The new species is characterised by
its medium body length (4.7-6.6 mm), lip region anteriorly flattened,
set-off from the rest of the body by a slight depression, pocket-shaped
amphidial pouch, odontostyle 84-98 µm and tail conical with bluntly
rounded tip. Males are present in the population in the ratio 1:1 and have
short spicules 39-49 µm. Canonical discriminant analysis distinguished
this new species from populations of L. elongatus and L. attenuatus.
Key words: Longidorus artemisiae sp. n., L. elongatus,
L. attenuatus, Russia, morphometrics, canonical discriminant analysis.
S.E. and Moens, M.
Two previously unreported species of steinernematids
from woodlands in Belgium
Two steinernematid species, Steinernema kraussei and Steinernema
sp. E1, which were not reported during previous surveys in Belgium, were
recovered from woodland soils by direct extraction of infective stages
from soil samples. Identification of the juveniles was based on their morphology
and confirmed by RFLP analysis of the PCR amplified ITS-rDNA region. Baiting
by Galleria mellonella larvae of soil samples in which these two
species were present, did not result in the normal infection development
and was not followed by juvenile migration. It is concluded that the presence
of some populations of these two species in soil may not be revealed by
Key words: Steinernematidae, Belgium, Galleria
D. and Ruess, L.
An undescribed Steinernema sp. (Nematoda: Steinernematidae)
from Germany and the Scandinavian Subarctic
Infective-stage juveniles of an undescribed Steinernema species
were isolated by direct extraction from soil samples in Germany. The samples
originated from five non-arable sites in the northern region of the country.
The morphology of the juveniles resembled that of S. intermedium
and S. affine. Steinernema juveniles isolated from a subarctic
heath in northern Sweden appear to belong to the same undescribed species.
The potential hosts appear to prefer natural, non-arable, moist habitats.
Key words: Arctic, entomopathogenic nematode, Germany,
soil, Steinernema, Sweden
S.E., Hominick, W.M. and Briscoe, B.R.
Morphology of amoeboid cells in the uterus of Steinernema
Amoeboid cells of different morphology and behavior were observed in
the lumen of the uterus of giant females of Steinernema spp. Cells
from 13 described species (S. affine, S. arenarium, S. bicornutum, S.
carpocapsae, S. ceratophorum, S. cubanum, S. glaseri, S. feltiae, S. intermedium,
S. karii, S. kraussei, S. longicaudatum and S. riobrave) and
6 undescribed species (British isolates C1 and B3, Sri-Lankan isolates
SSL1 and SSL2 and Japanese isolates MY1 and MY5) were studied. These amoeboid
cells are similar in their the morphology to the spermatozoon in the nematode
elegans, lending support to the suggestion that they are also spermatozoa.
Morphologically similar and related species of steinernematids are characterized
by having similar structure and behaviour of their associated amoeboid
Key words: Steinernema spp., taxonomy, in uteri
structures, amoebid cells, rhabditid spermatozoon
S.A., Halford, P.D. and Perry, R.N.
Identification of populations of potato cyst nematodes
from Russia using protein electrophoresis, rDNA-RFLPs and RAPDs
Twenty nine potato cyst nematode populations from Russia, five populations
from England and one population each from the Ukraine, New Zealand, Germany
and The Netherlands were compared using protein electrophoresis, restriction
fragment length polymorphism of ribosomal DNA (rDNA-RFLP) and random amplified
polymorphic DNA (RAPD) techniques. All populations from Russia were identified
as Globodera rostochiensis and RAPD analysis revealed substantial
Key words: molecular taxonomy, RAPD, rDNA-RFLP, Globodera
rostochiensis, Russia, protein electrophoresis, identification