Volume 5, Part 1 (1997)
on the variability in reproduction of some populations of root-knot nematodes
(Meloidogyne spp.) on resistant tomatoes in Crete, Greece, 1-6
& R. Peña-Santiago. Nematodes of the order Dorylaimida from Andalucia
Oriental, Spain. The genus Nygolaimus Cobb, 1913. II. Description
of two new species, 7-16
Guirado & M. Escuer. Mononchid nematodes from Spain. A description
Anatonchus acutus Altherr, 1974, 17-22
M.I. Rudenko, V.P. Balakhnina & V.A. Pukhalskiy. The selection
of homozygous lines of barley resistant to Heterodera filipjevi
based on the nematode resistant Turkish k-6808 cultivar, 23-26
& T.V. Volkova.Two new species of Criconemella (Tylenchida:
Criconematidae) from meadows in the Primorsky region, Russian Far East, 27-30
E.M. Kozodoi, A.P. Reid & S.E. Spiridonov. Redescription of
arenarium (Artyukhovsky, 1967) topotypes from Central Russia and a
proposal for S. anomalae (Kozodoi, 1984) as a junior synonym, 31-37
& R. Peña-Santiago. Nematodes of the order Dorylaimida from
Andalucia Oriental, Spain. The genus Nygolaimus Cobb, 1913. III.
Description of N. diversus sp. n., 39-43
Ley & D.L. Coyne. Actinca irmae sp. n., a new species
of Actinolaimidae (Nematoda: Dorylaimida) from the Ivory Coast, 45-54
L. Waeyenberge & M. Moens. Natural distribution of entomopathogenic
nematodes (Heterorhabditidae and Steinernematidae) in Belgian soils, 55-65
Miduturi, R. Moermans & M. Moens. Distribution of entomopathogenic
nematodes in a grassland, 67-70
N.I. Sumenkova & T.M. Gennadieva. Nematode types in the collection
of the Phytonematology Laboratory at the Institute of Parasitology of the
Russian Academy of Sciences, 71-74
- Book Reviews
Observations on the variability in reproduction of
some populations of root-knot nematodes (Meloidogyne
spp.) on resistant tomatoes in Crete, Greece.
Experiments under controlled conditions with a few naturally occurring
populations and single egg mass lines of root-knot nematodes (Meloidogyne
spp.) from Crete demonstrated the nematodes ability to produce low numbers
of egg masses on several resistant tomato cultivars. Except for one naturally
virulent population of M. javanica, reproduction on resistant cultivars
was significantly lower than on a susceptible cultivar. A quantitative
nature of the Mi resistance gene was revealed by the differential reproduction
of a partially virulent line of M. javanica on heterozygous and
homozygous resistant tomatoes. Virulent clones were not produced from a
few single egg mass lines of Meloidogyne spp. on a resistant cultivar
during a two generation period. Only one line produced two egg masses for
two generations but the evidence is probably insufficient to prove a genetic
inheritance of virulence. At high soil temperature (>28 C) resistance was
probably decreased and a non-virulent population reproduced on a resistant cultivar. The requirements for further research and practical
recommendations for successful implementation of resistant tomatoes in
management strategies are discussed.
Key words: Lycopersicon esculentum, Mi gene, Meloidogyne
javanica, M. incognita, plant resistance, root-knot nematodes,
G. & Peña-Santiago, R.
Nematodes of the order Dorylaimida from Andalucia Oriental,
Spain. The genus Nygolaimus Cobb, 1913. II. Description of two new
Two new species of the genus Nygolaimus Cobb, 1913 are described
from material collected in different natural areas from southeastern Spain.
baeticus sp. n. is characterized by its slender (a=47.6-64.1), long
(L=2.3-3.2 mm in females and L=1.8-2.5 mm in males) body, lip region set-off
by constriction, mural tooth deltoid and 11-13.5 µm long at the dorsal
side or about four-fifths of the lip region width, V=53.3-59.7%, tail rounded,
conoid (31-42 µm, c=71.4-96.5 and c'=0.8-1.1 in females; 27-44 µm, c=54.5-81.3
and c'=0.9-1.4 in males), spicules 37-42 µm long and one or no ventromedian
supplement. Nygolaimus similis sp. n. can be distinguished by its
relatively slender (a=40-57.7), long (L=2.4-3.2 mm in females and L=1.6-1.8 mm in males) body,
lip region 18-21 µm and set-off by a deep constriction,
mural tooth deltoid and 14.5-17 um long at the dorsal side or about three-fourths
of the lip region diameter, V=48.5-55.3%, tail rounded, conoid (34-49 µm,
c=52.9-81.9, c'=1.0-1.3 in females; 34-36 µm, c=44.1-52.5, c'=1.1-1.2 in
males) with an indentation in the cuticle of the tail in the subterminal
dorsal position, spicules 38-39 um and no ventromedian supplement. Both
species are illustrated and tables of morphometrics are given.
Key words: Nygolaimus baeticus sp. n., Nygolaimus
similis sp. n., Spain
D. and Escuer, M.
Mononchid nematodes from Spain. A description of Anatonchus
acutus Altherr, 1974.
Females and juveniles of Anatonchus acutus Altherr, 1974 are
described and illustrated for the first time from specimens collected in
Spain. Male specimens are also described from the same populations. The
species is redefined and characterized by its medium size, lip region offset
by a strong depression, buccal cavity 23-32 x 28-42 µm, teeth located in
the anterior half of the stoma with the apex of the dorsal tooth at 46-63%
of the buccal cavity length measured from the base, V=67-73%, female genital
system pseudomonodelphic or didelphic with the posterior branch reduced,
ejaculatory glands in tandem, spicules 52-68 µm, lateral guiding pieces
bifurcate with straight arms, 9-13 ventromedian supplements, tail conical,
elongate with acute terminus and caudal glands or spinneret absent. It
is similar to A. amiciae Coomans & Lima, 1965 and A. monohystera
Altherr, 1977 in the characteristics of the female genital system but it differs in tail
shape and absence of caudal glands and spinneret.
Key words: Anatonchus acutus, Mononchida, Spain, taxonomy
Rudenko, M.I., Balakhnina, V.P. and Pukhalskiy, V.A.
The selection of homozygous lines of barley resistant
to Heterodera filipjevi based on the nematode resistant Turkish k-6808 cultivar
New lines of barley with genetic resistance against Heterodera filipjevi
were produced from crossing the high yielding but nematode susceptible
cultivar Donetsky 4 with the low yielding but highly nematode resistant
Turkish k-6808 cultivar. The Turkish cultivar of barley was used here for
the first time as a source of resistance against the nematode. The yield
characteristics of the new barley lines were comparable with that of Zazersky
85, the most widely grown cultivar in the former Soviet Union.
Key words: Heterodera filipjevi, barley, resistance,
Eroshenko, A.S. and
Two new species of Criconemella (Tylenchida:
Criconematidae) from meadows in the Primorsky region, Russian Far East
Two new species Criconemella ripariensis sp. n. and C. sigillaria
sp. n. are described from specimens collected from the rhizosphere of Carex
appendiculata and C. lasiocarpa growing in meadows in the Khassan
district of the Primorsky region, Russia. Criconemella ripariensis
sp. n. is characterized by the presence of 125-150 cuticular annules, stylet
64-73 µm long, conical tail curved on the dorsal side and deformed annules
on the tail terminus.
C. sigillaria sp. n. is characterized by the
presence of 115-127 cuticular annules with anastomoses, stylet 46-51 µm
long and conical tail with dorsally curved terminus with numerous finger-like
appendages. Macroposthonia digiticauda Eroshenko & Volkova,
1988 is transferred to Criconemella digiticauda (Eroshenko &
Volkova, 1988) comb. n.
Key words: Criconemella ripariensis sp. n., C.
sigillaria sp. n., taxonomy, Russian Far East.
A.K., Kozodoi, E.M., Reid, A.P. and Spiridonov, S.E.
Redescription of Steinernema arenarium (Artyukhovsky,
1967) topotypes from Central Russia and a proposal for S. anomalae
(Kozodoi, 1984) as a junior synonym.
A redescription of Steinernema arenarium (Artyukhovsky, 1967)
Poinar 1990 is presented based on topotypes collected from the type locality
in the Voronezh, Central Russia. The synonymization of
(Kozodoi, 1984) Curran, 1989, originally described from nematodes collected
in the Ryazan region, Central Russia, with S. arenarium is proposed
based on morphometrics, protein electrophoresis and DNA analysis.
Key words: entomopathogenic nematodes, Steinernema
arenarium, S. anomalae, synonymization, Central Russia, redescription.
G. and Peña-Santiago, R.
Nematodes of the order Dorylaimida from Andalucia Oriental,
Spain. The genus Nygolaimus Cobb, 1913. III. Description of N.
diversus sp. n.
Nygolaimus diversus sp. n. is described from natural biotopes
in southeastern Spain. It is characterized by its medium body length (L=1.3-2.0
mm), asymmetrical lip region 12.5-15.0 µm wide offset by a weak constriction,
deltoid mural tooth with its dorsal side 10.5-12.0 µm long or two-thirds
to four-fifths of the lip region width, V=53-59%, rounded conoid tail (25-32
µm, c=43-63 and c'=1.1-1.4), makes unknown and females not containing sperm.
Illustrations and a table of morphometrics are provided.
Key words: Nygolaimus diversus sp. n., Spain, taxonomy.
De Ley, P. and Coyne, D.L.
Actinca irmae sp. n., a new species of Actinolaimidae
(Nematoda: Dorylaimida) from Ivory Coast.
Actinca irmae sp. n. was found in three soil samples from rice
fields in Gagnoa, Ivory Coast. It is characterized by having a peg-like
cuticular ornamentations anteriorly in the neck region and in having males
with a total of 20-26 pseudosupplements, including two subventral rows
of 5-7 pseudosupplements lying anterior to the first fascicle. The structure
of the cheilostom is also distinctive, having a post-onchial part that
is strongly constricted just anterior to the guiding ring and is 11-13
um long, distinctly sclerotized and expanded over most of its length. The
new species is further characterized by a relatively well-developed vestibular
ring, a slightly expanded lip region that is wider than high, a slender
27-31 um long odontostyle and 14 cuticular ridges. As A. irmae sp.
n. combines features of the genera or subgenera Actinca and Stomachoglossa
it is proposed that the latter be considered a junior synonym of the former.
Key words: Actinca irmae sp. n., Ivory Coast, taxonomy,
J.S., Waeyenberge, L. & Moens, M.
Natural distribution of entomopathogenic
nematodes (Heterorhabditidae and Steinernematidae) in Belgian soils.
In eight provinces of Belgium, 248 soil samples were collected in 72
ecologically diverse habitats to isolate indigenous entomopathogenic nematodes
(epns). Using the Galleria larva bait technique, 21 soil samples
were found positive for epns. The morphometric study of infective juveniles
(IJs) classified 20 populations as Steinernema spp., and one population
as Heterorhabditis sp. Further biochemical characterization differentiated
eight Steinernema feltiae A1 type, eight S. affinis, four
sp. B3 and one Heterorhabditis megidis (northwest European type).
The entomopathogenic nematodes were recovered from 38.1%, 28.5%, 23.8%
and 9.6% of the samples taken in woodland, roadside verges, cultivated
land and grassland respectively. Steinernema feltiae A1 type was
isolated in all the habitats except in roadside verges, whereas,
affinis was not found in cultivated land. Steinernema sp. B3
was isolated from cultivated fields and roadside verges; H. megidis from grassland. Steinernema feltiae was prevalent in sand to loamy
sand soils with a wide range of organic matter content. Steinernema
affinis was recovered in sand and sandy clay loam soils. Heterorhabditis
sp. and Steinernema sp. B3 were isolated in sandy loam and loam
soils with organic matter content of 5.6% and 7.5% respectively. All isolates
were found in soils with a pH range of 3.6-7.8. However, most of the S.feltiae
populations were isolated in acidic soils.
Key words: entomopathogenic nematodes, morphometrics, habitat,
soil type, RFLPs
J.S., Moermans, R. and Moens, M.
Distribution of entomopathogenic nematodes in a grassland
The distribution of Steinernema feltiae and Heterorhabditis
megidis (NWE type) in a grassland habitat was studied during autumn
1994. Larvae of the wax moth, Galleria mellonella, were used as
bait. Results indicated an aggregated distribution for both species. Sand
fraction and organic matter content did not always influence the distribution
of the nematodes. Largest numbers of S. feltiae were detected in
samples with a sand fraction content higher than 90%. The area in which
megidis was detected, was somewhat limited and had a sand fraction
greater than 94%. Numbers of S. feltiae were negatively influenced
by higher organic matter content.
Key words: aggregation, granulometry, organic matter