Volume 17, Part 1 (2009)
Using ecological niche modelling to predict geographical expansion of an invasive species Bursaphelenchus xylophilus in Asia, 1-11
K. Zoltowska, E. Lopienska-Biernat and J. Rokicki.
Activity of glycogen catabolism enzymes in Contracaecum rudolphii (Anisakidae), 11-16
N.P. Fadeeva and S.I. Maslennikov.
The abundance and distribution of free-living marine nematodes of the Piltun-Astokhskoye oil and gas field (North-East Sakhalin), 31-42
O. Holovachov, I. Tandingan De Ley, M. Mundo-Ocampo, R. Gingold and P. De Ley. Part 3. Three new species of the genus Diplopeltoides Gerlach, 1962 (Nematoda: Diplopeltoididae) with overviews of the genera Diplopeltis Gerlach, 1962 and Dplopeltula Gerlach, 1950, 43-58.
S. Ya. Tsalolikhin and A. V. Shoshin. Review of the genus Neotobrilus Tsalolikhin, 1981 (Nematoda, Enoplida: Tobrilidae), 59-72
P. Q. Trinh, E. de la Pena, C. N. Nguyen, H. X. Nguyen and M. Moens. Plant-parasitic nematodes associated with coffee in Vietnam, 73-82
- J. Karimi, A. Kharazi-Pakdel, T Yoshiga and M. Koohi-habibi.
Report of entomopathogenic nematode, Steinernema glaseri, from Iran, 83-86
Newsletter of the Russian Society of Nematologists
Using ecological niche modelling to predict geographical expansion of an invasive species Bursaphelenchus xylophilus in Asia
The potential distribution of Bursaphelenchus xylophilus in Asia was investigated through ecological
niche modelling. The modelling procedure was developed based on night environmental variables.
Principal component analysis was used to describe environmental niche differentiation of the species. The
analysis identified temperature and precipitation as the most important factors affecting the invasiveness
of B. xylophilus. This implied that water and energy played the dominant role in the potential spreading
of B. xylophilus. No significant relationship was found between human population density and environmental
niches exhibited by B. xylophilus. Further monitoring effect should be enhanced in those natural
landscapes with moderate temperature and precipitation, for example, temperate zone in China, Japan
and Korea. Areas with relatively low precipitation would suffer higher risk of the species as indicated by
ecological niche visualization. Some areas in SW China will become highly susceptible to the species in
the 2050 because of climate change. Controlling pest species Monochamus alternatus was a highly efficient
surrogate for controlling B. xylophilus in the fieldwork. My findings contribute to further understanding
the invasive ecology of B. xylophilus and have implications in sustainable integrated pest management
Key words: Bursaphelenchus xylophilus; ecological niche modeling; GARP model; human population
density; invasive species; principal component analysis.
Zoltowska K., Lopienska-Biernat E. and Rokicki J.
Activity of glycogen catabolism enzymes in Contracaecum rudolphii (Anisakidae)
Extracts of Contracaecum rudolphii L3 and L4 larvae, females and males were assayed for
carbohydrate content and activities of enzymes that decompose glycogen and disaccharides. The sugar
content was about 6-8% of nematode wet weight. In terms of their content, the saccharides assayed could
be arranged in the following order: glycogen, glucose and trehalose. All carbohydrate concentrations were
greater in females than in males. C. rudolphii shows activity of enzymes that decompose glycogen via
phosphorolysis and hydrolysis. A high activity of glycogen phosphorylase (2.89 mmol mg-1) was recorded
in the L3 larvae; the activity observed in the adults was lower by half. The amylase activities in all the
forms of the parasite were relatively high and practically the same (0.163-0.175 U mg-1 and 0.93-1.03
µmol mg-1 for a-amylase and glucoamylase, respectively). Among disaccharidases (maltase, saccharase,
lactase), the greatest activity was shown by maltase. It was particularly high (2.67 µmol mg-1) in the L3.
The saccharase activity in these larvae was twice that of the adults. Lactase had the lowest activity (0.26-
0.49 µmol mg-1).
Key words: Anisakidae; Contracaecum rudolphii; enzymes; metabolism of glycogen.
Fadeeva N.P. and Maslennikov S.I.
The abundance and distribution of free-living marine nematodes of the Piltun-Astokhskoye oil and gas field (North-East Sakhalin)
Although offshore oil and gas production platforms are a potential source for a number of
toxic compounds, their impact on the meiofauna is insufficiently studied. In the vicinity of the oil and
gas production platform MOLIKPAQ (North-East Sakhalin, the Sea of Okhotsk), the relative abundance
of meiofauna assemblages ranged from 23.5 to 406.0 ind. 10 cm-2. The dominant group, nematodes,
ranged from 6 to 385 ind. 10 cm-2. The maximum nematode density was at the distance of 250 m from
the platform coupled with the highest petroleum hydrocarbons concentration (6.52 mg g-1) and the
lowest values of the Shannon-Wiener diversity and PielouŐs evenness indices (1.25 and 0.33,
respectively). In total, 69 nematode species were found. Disturbance in the structure of nematocenes,
decrease of number species, drop of biological diversity indices, as well as increase in dominance of some
nematode species of occurred in response to the introduction of PHCs as a stress factor. CLUSTER,
MDS and SIMPER analyses clearly distinguished site groupings related to the different concentrations of
PHC and sediment granulometry. The significant differences of nematode assemblages occurred in the
response to the appearance of even low-level PHC contamination sediment. These results suggest that
nematode assemblages can be used to detect non-catastrophic levels of oil contamination.
Key words: Free-living marine nematodes, meiofauna, petroleum hydrocarbons, the Sea of Okhotsk.
Key words: Free-living marine nematodes, meiofauna, petroleum hydrocarbons, the Sea of Okhotsk.
Holovachov O., Tandingan De Ley I., Mundo-Ocampo M., Gingold R., and De Ley, P.
Nematodes from the Gulf of California. Part 3. Three new species of the genus Diplopeltoides Gerlach, 1962 (Nematoda: Diplopeltoididae) with overviews of the genera Diplopeltis Gerlach, 1962 and Diplopeltula Gerlach, 1950
Three new species, Diplopeltoides axayacatli sp. n., D. santaclarae sp. n. and D. paramastigia
sp. n., are described using light microscopy. All three species are morphometrically close to D. mastigia
but the former differs in having a hook-shaped amphid, while the latter two can be distinguished from D.
mastigia by having a gubernaculum with apophysis, and from each other by having, respectively, a
striated cuticle (versus smooth) and a punctated interamphidial shield (versus crenate). The status and
composition of Diplopeltoides is discussed and compared with those of Diplopeltis and Diplopeltula. The
following species are transferred to different genera: Diplopeltula curta is placed in Pararaeolaimus;
Diplopeltis bathmanni is placed in Diplopeltula; Diplopeltula pulcher and D. sundensis are transferred to
Diplopeltoides. Three species of Diplopeltula are considered species incertae sedis: D. botula, D. striata and
Key words: Baja California, Diplopeltis, Diplopeltoides axayacatli sp. n., Diplopeltoides paramastigia sp. n.,
Diplopeltoides santaclarae sp. n., Diplopeltula, morphology, morphometrics, SEM, taxonomy.
Tsalolikhin S. Ya. and ShoshinA. V.
Review of the genus Neotobrilus Tsalolikhin, 1981 (Nematoda, Enoplida: Tobrilidae)
The composition of the genus Neotobrilus was revised and new synonyms were established. Descriptions of a new species Neotobrilus sinensis sp.n. from Taiwan and a number of species from new localities are given. Some morphological characters as taxonomic ones are proposed. A species
identification key is given.
Key words: morphology, morphometric, Neotobrilus identification key, Neotobrilus sinensis sp.n.,
Trinh P. Q., la Pena E. de, Nguyen C. N., Nguyen H. X. and Moens M.
Plant-parasitic nematodes associated with coffee in Vietnam
Roots and soil were sampled in May 2005 from coffee plantations in fifteen localities of the
seven coffee producing provinces of Vietnam for the presence of plant-parasitic nematodes on Coffea
arabica and C. canephora. Twenty-one plant-parasitic nematodes species representing 14 genera were
recovered from the rhizosphere soil. The endoparasitic nematodes Rotylenchulus reniformis, Meloidogyne
spp., P. coffeae and R. arabocoffeae were predominant and occurred in 22%, 21%, 27% and 11% of all
soil samples with 332, 257, 124 and 61 individuals/250 cm3 soil, respectively. Pratylenchus coffeae, R.
arabocoffeae and Meloidogyne spp. were found in 24%, 9% and 12% of all root samples, respectively.
Pratylenchus coffeae was present in root samples from 11 of the 15 localities, while Meloidogyne occurred
in root samples from 9 localities but was present in soil samples from 12 localities. Root-knot nematodes
were not found in root and soil samples from the Northern region (Muong Ang and Son La) and Dak
Doa. Radopholus arabocoffeae was not detected in samples from localities from Northern and Centre of
Vietnam, but was only present in some of the Western Highlands localities. Plant-parasitic species
recorded for the first time in Vietnam in association with coffee were Hoplolaimus chambus, Xiphinema
elongatum, Diphterophora perplexans, Apratylenchus vietnamensis and A. binhi. The occurrence of the main
nematode genera varied across clusters of soil texture. Samples with high numbers of Meloidogyne spp.
were grouped in clay soils, whereas R. arabocoffeae occurred in sandy and loamy soils indistinctively.
Pratylenchus spp. and R. reniformis were present in large numbers in sandy soils.
Key words: Coffea arabica, Coffea canephora, endoparasitic nematodes, soil characteristics, plant age.