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Volume 11, Part 1 (2003)

  • S. Chen, X. Han and M. Moens. Effect of chlorpyrifos on infectivity and survival of Steinernema feltiae, 1-6
  • M. Ciobanu, I. Popovici and R. Pea-Santiago. Nematodes of the order Dorylaimida from Romania. Contribution to the knowledge of the genus Tylencholaimellus Cobb in M. V. Cobb, 1915 , 7-16
  • J. Zheng, S.A. Subbotin, S. He, J. Gu, M. Moens. Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA, 17-22
  • O. Holovachov. Redescription of Anguinoides stylosum Chitwood, 1936 (Nematoda: Leptolaimidae), 23-25  
  • Y. Aguirre, R. Crozzoli and N. Greco. Effect of the root-knot nematode Meloidogyne incognita on parsley, 27-31
  • J. Abolafia and R. Pea-Santiago. Nematodes of the order Rhabditida from Andaluca Oriental, Spain. The genus Stegelletina Andrssy, 1984 with description of two new species, 33-49
  • S. G. Sokolov. First record of the genus Esocinema Moravec, 1977 (Spirurida, Skrjabillanidae) in pike, Esox lucius L, in Russia, 51-52
  • L.A. Pylypenko and D.D. Sigareva. The distribution of the sugar beet nematode (Heterodera schachtii) in the Ukraine, 53-55
  • M. A. Ansari, L. Phan Ke and M. Moens. Heterorhabditis bacteriophora (Heterorhabditidae: Rhabditida), parasitic in natural populations of white grubs (Coleoptera: Scarabaeidae) in Belgium, 57-59
  • A. Stanelis. The occurrence of Longidorus euonymus Mali & Hooper, 1974 (Nematoda: Dorylaimida) in Lithuanian natural grassland, 61-62

Chen, S., Han, X. and Moens, M.

Effect of chlorpyrifos on infectivity and survival of Steinernema feltiae

Summary:
The effect of chlorpyrifos on the mortality and the infectivity of Steinenema feltiae was tested in suspension or sand assays. The nematode response was significantly influenced by exposure times and insecticide doses as well as by their interaction. Although in the suspension assay the nematode mortality was relatively unaffected, the nematode infectivity was highly suppressed. In the sand assay, however, both the mortality and infectivity of the nematodes were significantly suppressed. Overall results indicate chlorpyrifos and S. feltiae cannot be used together in insect control strategies.

Key words: compatibility, entomopathogenic nematodes, Galleria mellonella, insecticide.


Ciobanu, M., Popovici, I. and Pea-Santiago, R.

Nematodes of the order Dorylaimida from Romania. Contribution to the knowledge of the genus Tylencholaimellus Cobb in M. V. Cobb, 1915)

Summary:
Specimens belonging to four species of Tylencholaimellus (T. grandis Thorne, 1974, T. eskei Siddiqi & Khan, 1964, T. paracinctus Peralta & Pea-Santiago, 1996 and T. polonicus Szczygiel, 1962) collected from various localities in Romania were studied by light microscope. New data referring to the distribution in additional habitats of T. eskei are provided. Tylencholaimellus grandis, T. paracinctus and T. polonicus are new records for the Romanian nematode fauna. The identity of T. grandis is revalidated and the species is reported for the first time in Europe. Descriptions, measurements, illustrations and distribution data are provided.

Key words: description, nematodes, Romania, taxonomy, Tylencholaimellus.


Zheng, J., Subbotin, S.A., He, S., Gu, J., Moens, M.

Molecular characterisation of some Asian isolates of Bursaphelenchus xylophilus and B. mucronatus using PCR-RFLPs and sequences of ribosomal DNA

Summary:
Five isolates of Bursaphelenchus xylophilus, four of which were collected from Chinese forests and one from Japan, as well as, four isolates of B. mucronatus extracted from pinewood packaging imported from Japan, Korea and from forests in China, were studied using PCR-RFLPs and sequences of the ITS and D2-D3 fragments of rDNA. RFLP analysis of the ITS region with restriction endonucleases revealed that CfoI, HaeIII, HinfI, MspI and TaqI yielded distinct patterns that separated B. xylophilus and B. mucronatus. Phylogenetic analyses of alignments of published and original sequences of both the ITS1 region and ITS1-5.8S-ITS2 region revealed that both B. xylophilus and B. mucronatus are composed of two groups of populations with different ITS types. The taxonomic status of these groups is discussed.

Key words:  28S rDNA, Bursaphelenchus, China, ITS-rDNA, Japan, phylogenetic analysis.


Holovachov, O.

Redescription of Anguinoides stylosum Chitwood, 1936 (Nematoda: Leptolaimidae)

Summary:
Anguinoides stylosum Chitwood, 1936 is redescribed from type material. New data on the morphology of the species as well as emended diagnosis of the genus Anguinoides Chitwood, 1936 are given.

Key words: Leptolaimidae, morphology, redescription.


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Aguirre, Y., Crozzoli, R. and Greco, N.

Effect of the root-knot nematode Meloidogyne incognita on parsley

Summary:
A screen house experiment was conducted in 1000 cm3 plastic pots to evaluate the effect of increasing population densities of M. incognita race 1 on yield of parsley cv. Double Curled. Initial nematode densities were: 0, 0.125, 0.25, 0.5, 1, 2, 4, 8, 16, 32, 64, 128 and 256 eggs + second stage juveniles (J2) cm-3 soil. Fitting the Seinhorst model, y = m+(1-m)zPi-T, to average plant top and total fresh weight and top dry weight, gave tolerance limits (T) of 0.17, 0.02 and 0.025 eggs + J2 cm-3 soil. The minimum relative yields (m) were 0.5 at Pi > 16 eggs + J2 cm-3 soil, 0.55 at Pi > 2 eggs + J2 cm-3 soil and 0.34 at Pi > 4 eggs + J2 cm-3 soil for top fresh weight, total fresh weight and top dry weight. The maximum nematode reproduction rate was 37-fold at Pi = 0.25 eggs + J2 cm-3 soil and an equilibrium density was estimated at 5 eggs + J2 cm-3 soil. Anatomical modifications observed in galled roots were similar to those induced by this root-knot nematode species in other hosts.

Key words: Meloidogyne incognita, parsley, pathogenicity, Petroselinum sativum, root-knot nematode, Venezuela.


Abolafia, J. and Pea-Santiago, R.

Nematodes of the order Rhabditida from Andaluca Oriental, Spain. The genus Stegelletina Andrssy, 1984 with description of two new species

Summary:
Two new and two known species of the genus Stegelletina are described from natural areas of SE Iberian Peninsula. Stegelletina pigmaea sp. n. is characterized by a body size of 364-401 m in females and 376-415 m in males, lateral field with three incisures, lips bearing four tines, labial probolae 3 m long and bifurcate, pharyngeal corpus 1.9-2.4 times isthmus length, spermatheca 15-37 m long, postuterine sac 0.5-1.2 times the corresponding body diameter long, female tail conical (22-27 m, c=13.7-17.1, c'=1.8-2.3) with acute or rounded mucro, male tail conical with minutely rounded or acute mucro (17-27 m, c=14.2-22.8, c'=1.2-1.8), spicules 19-21 m long, and gubernaculum 10 m long. Stegelletina salinaria sp. n. is distinguished by a body size of 311-377 m in females, lateral field with three incisures, lips with three lobes, labial probolae 4-5 m long and bifurcate, pharyngeal corpus 2.0-3.4 times isthmus length, spermatheca 12-16 m long, postuterine sac 0.3-0.9 times the corresponding body diameter long, female tail conical (20-28 m, c=13.1-17.7, c'=1.7-2.3), and phasmid at 32-39% of tail length. Descriptions, measurements and illustrations, including SEM photographs, are provided for the four species. Finally, a key to species identification is also given.

Key words: Cephalobidae, description, key, morphology, new species, Stegelletina, Spain, SEM, taxonomy.